Journal: Frontiers in Endocrinology
Article Title: 17β-Estradiol Induces Mitophagy Upregulation to Protect Chondrocytes via the SIRT1-Mediated AMPK/mTOR Signaling Pathway
doi: 10.3389/fendo.2020.615250
Figure Lengend Snippet: 17β-E2 treatment upregulated the phosphorylation level of LC3, TOM20, and Hsp60 in ATDC5 chondrocytes. The chondrocytes were pretreated with 15 μM NAM or 20 μM Compound C for 30 min and then incubated with or without 1 × 10 -7 M 17β-E2 for 24 h. The phosphorylation levels of (A) microtubule-associated protein 1A/1B light chain 3 (LC3), (B) translocase of outer membrane 20 (TOM20), (C) heat shock protein 60 (Hsp60), and β-actin protein were detected by Western blot analysis. These experiments were independently repeated 3 times. * P < 0.05 versus the control group indicated a significant difference.
Article Snippet: The polyclonal goat anti-mouse heat shock protein 60 (Hsp60) antibody (sc-1052) (1:200), the monoclonal mouse anti-mouse microtubule-associated protein 1A/1B-light chain 3 (LC3) antibody (sc-376404) (1: 100), rabbit monoclonal anti-β-actin and the polyclonal rabbit anti-mouse primary translocase of outer membrane (TOM) 20 antibody (sc-11415) (1: 200) were purchased from Sigma (MO, USA).
Techniques: Incubation, Western Blot